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global dna methylation line 1 kit  (Active Motif)


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    Active Motif global dna methylation line 1 kit
    Global Dna Methylation Line 1 Kit, supplied by Active Motif, used in various techniques. Bioz Stars score: 95/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/global+dna+methylation/Global+DNA+Methylation+-+LINE-1+Kit/pmc13006433-413-7-13
    Average 95 stars, based on 67 article reviews
    global dna methylation line 1 kit - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    DNA Methylation Assay:

    Article Title: Targeting heterochromatin eliminates chronic myelomonocytic leukemia malignant stem cells through reactivation of retroelements and immune pathways
    Article Snippet: Genomic DNA quality and quantity were measured using the NanoDrop (ND-1000, Marshall Scientific). .. Global DNA methylation was studied using the global DNA methylation assay kit—LINE-1 (Active Motif) according to the manufacturer’s instructions. .. A total of 5000 human and murine HSPCs/HSCs were fixed with 4% paraformaldehyde (PFA) for 10 min at RT and cytocentrifuged (500 × g , 5 min; Tharmac) on poly-L-Lysine slides (Fisher Scientific).

    Article Title: Inhibition of DNMT1 methyltransferase activity via glucose-regulated O -GlcNAcylation alters the epigenome
    Article Snippet: .. Global DNA methylation of LINE-1 elements was measured via kit (Active Motif, Cat #55017, Carlsbad, CA) according to the manufacturer’s instructions. ..

    Article Title: Mapping of DNA methylation-sensitive cellular processes in gingival and periodontal ligament fibroblasts in the context of periodontal tissue homeostasis
    Article Snippet: Genomic DNA was extracted using a DNeasy Blood and Tissue Kit (Qiagen) and quantified with a BioPhotometer D30. .. Global DNA methylation was assessed using a Global DNA Methylation LINE-1 Kit (Active Motif), according to the protocol provided by the manufacturer. ..

    Article Title: Betaine supplementation improves positive symptoms in schizophrenia.
    Article Snippet: Because the CHDH variant rs35518479 affected CHDH expression levels and methylation index in postmortem brain tissues (Ohnishi et al., 2019), we analyzed a single nucleotide polymorphism of CHDH, rs35518479, as a potential biomarker for stratifying patients with schizophrenia. .. We also analyzed global DNA methylation using a Global DNA Methylation LINE-1 Kit (Active Motif, California, USA) because global DNA methylation was correlated with plasma betaine in patients with schizophrenia (Murata et al., 2020). ..

    Article Title: Targeting Heterochromatin Eliminates Malignant Hematopoietic Stem and Progenitor Cells in Chronic Myelomonocytic Leukemia Through Reactivation of Retroelements and Innate Immune pathways
    Article Snippet: Genomic DNA quality and quantity were measured using the NanoDrop (ND-1000, Marshall Scientific). .. Global DNA methylation was studied using the global DNA methylation assay kit - LINE-1 (Active Motif) according to the manufacturer’s instructions. .. Dry pellets of 10,000 CD34 + cells stored at −80°C were resuspended in Laemmli sample buffer (ref. 1610747, bioRad®), and incubated at 95°C for 10 min. Denatured protein extracts were separated on a 15% polyacrylamide-SDS gel and transferred to a nitrocellulose membrane (ThermoFischer Scientific).

    Article Title: LINE-1 ORF1p as a candidate biomarker in high grade serous ovarian carcinoma.
    Article Snippet: .. LINE-1 methylation was assessed using the Global DNA Methylation— LINE-1 Kit (Active Motif, Cat. #55017) following the manufacturer’s recommendations. ..

    Clinical Proteomics:

    Article Title: Betaine supplementation improves positive symptoms in schizophrenia.
    Article Snippet: Because the CHDH variant rs35518479 affected CHDH expression levels and methylation index in postmortem brain tissues (Ohnishi et al., 2019), we analyzed a single nucleotide polymorphism of CHDH, rs35518479, as a potential biomarker for stratifying patients with schizophrenia. .. We also analyzed global DNA methylation using a Global DNA Methylation LINE-1 Kit (Active Motif, California, USA) because global DNA methylation was correlated with plasma betaine in patients with schizophrenia (Murata et al., 2020). ..

    Methylation:

    Article Title: LINE-1 ORF1p as a candidate biomarker in high grade serous ovarian carcinoma
    Article Snippet: .. LINE-1 methylation was assessed using the Global DNA Methylation—LINE-1 Kit (Active Motif, Cat. #55017) following the manufacturer’s recommendations. ..

    Article Title: LINE-1 ORF1p as a candidate biomarker in high grade serous ovarian carcinoma.
    Article Snippet: .. LINE-1 methylation was assessed using the Global DNA Methylation— LINE-1 Kit (Active Motif, Cat. #55017) following the manufacturer’s recommendations. ..



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    HELLS promotes cancer cell survival and genome stability in response to <t>DNA</t> alkylation damage. (A) Western blot of Whole Cell Extract (WCE) in HAP1 and HELLS KO probed for HELLS. Actin was used as a loading control. (B) Schematic representation of survival assays using crystal violet staining. (C–F) Quantification of crystal violet cell survival for (C) MMS, (D) Temozolomide, (E) Bleomycin, or (F) Etoposide. Data are mean ± SEM. n = 3 independent biological replicates. Two-way ANOVA with Tukey’s multiple comparisons was used to determine significance. (G) HAP and HELLS KO sensitivity to Topotecan was determined by cck-8 after treatment with the indicated doses for 48 h. Data are mean ± SEM. n = 3 independent biological replicates. (H) Quantification of 5-methyl cytosine (5-mC) content of parental and HELLS KO cells of HAP1 and HeLa using the MethylFlash TM Global DNA <t>Methylation</t> <t>ELISA</t> kit. n = 4 independent biological replicates. Statistically significant differences were calculated using an unpaired t -test. I. Relative cell cycle distribution of cells after treatment with MMS. n = 3 independent biological experiments. (J) Representative image of micronuclei in HELLS KO cells. Bar = 20 µm (Left); 5 µm (Right). (K) Formation of micronuclei in response to MMS treatment. Micronuclei formation in HELLS KO HAP cells treated with MMS. Cells were treated with 250 or 500 μM MMS for 1 h. The number of micronuclei was visualized by DAPI staining in HAP1 control or HELLS KO HAP1 cells 48 h post MMS treatment. Micronuclei counts were performed in four microscopy fields in each experimental setting. Statistically significant differences were calculated using one-way ANOVA with Tukey’s multiple comparisons. Data are mean ± SEM and are representative of at least two independent experiments. (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).
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    Image Search Results


    HELLS promotes cancer cell survival and genome stability in response to DNA alkylation damage. (A) Western blot of Whole Cell Extract (WCE) in HAP1 and HELLS KO probed for HELLS. Actin was used as a loading control. (B) Schematic representation of survival assays using crystal violet staining. (C–F) Quantification of crystal violet cell survival for (C) MMS, (D) Temozolomide, (E) Bleomycin, or (F) Etoposide. Data are mean ± SEM. n = 3 independent biological replicates. Two-way ANOVA with Tukey’s multiple comparisons was used to determine significance. (G) HAP and HELLS KO sensitivity to Topotecan was determined by cck-8 after treatment with the indicated doses for 48 h. Data are mean ± SEM. n = 3 independent biological replicates. (H) Quantification of 5-methyl cytosine (5-mC) content of parental and HELLS KO cells of HAP1 and HeLa using the MethylFlash TM Global DNA Methylation ELISA kit. n = 4 independent biological replicates. Statistically significant differences were calculated using an unpaired t -test. I. Relative cell cycle distribution of cells after treatment with MMS. n = 3 independent biological experiments. (J) Representative image of micronuclei in HELLS KO cells. Bar = 20 µm (Left); 5 µm (Right). (K) Formation of micronuclei in response to MMS treatment. Micronuclei formation in HELLS KO HAP cells treated with MMS. Cells were treated with 250 or 500 μM MMS for 1 h. The number of micronuclei was visualized by DAPI staining in HAP1 control or HELLS KO HAP1 cells 48 h post MMS treatment. Micronuclei counts were performed in four microscopy fields in each experimental setting. Statistically significant differences were calculated using one-way ANOVA with Tukey’s multiple comparisons. Data are mean ± SEM and are representative of at least two independent experiments. (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

    Journal: Nucleic Acids Research

    Article Title: The chromatin regulator HELLS mediates SSB repair and responses to DNA alkylation damage

    doi: 10.1093/nar/gkaf1201

    Figure Lengend Snippet: HELLS promotes cancer cell survival and genome stability in response to DNA alkylation damage. (A) Western blot of Whole Cell Extract (WCE) in HAP1 and HELLS KO probed for HELLS. Actin was used as a loading control. (B) Schematic representation of survival assays using crystal violet staining. (C–F) Quantification of crystal violet cell survival for (C) MMS, (D) Temozolomide, (E) Bleomycin, or (F) Etoposide. Data are mean ± SEM. n = 3 independent biological replicates. Two-way ANOVA with Tukey’s multiple comparisons was used to determine significance. (G) HAP and HELLS KO sensitivity to Topotecan was determined by cck-8 after treatment with the indicated doses for 48 h. Data are mean ± SEM. n = 3 independent biological replicates. (H) Quantification of 5-methyl cytosine (5-mC) content of parental and HELLS KO cells of HAP1 and HeLa using the MethylFlash TM Global DNA Methylation ELISA kit. n = 4 independent biological replicates. Statistically significant differences were calculated using an unpaired t -test. I. Relative cell cycle distribution of cells after treatment with MMS. n = 3 independent biological experiments. (J) Representative image of micronuclei in HELLS KO cells. Bar = 20 µm (Left); 5 µm (Right). (K) Formation of micronuclei in response to MMS treatment. Micronuclei formation in HELLS KO HAP cells treated with MMS. Cells were treated with 250 or 500 μM MMS for 1 h. The number of micronuclei was visualized by DAPI staining in HAP1 control or HELLS KO HAP1 cells 48 h post MMS treatment. Micronuclei counts were performed in four microscopy fields in each experimental setting. Statistically significant differences were calculated using one-way ANOVA with Tukey’s multiple comparisons. Data are mean ± SEM and are representative of at least two independent experiments. (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

    Article Snippet: For Global 5-mC DNA methylation (5-mc) ELISA, genomic DNA was prepared from the parental and HELLS KO cells of HAP1 and HeLa using PureLink genomic DNA prep kit (Invitrogen).

    Techniques: Western Blot, Control, Staining, CCK-8 Assay, DNA Methylation Assay, Enzyme-linked Immunosorbent Assay, Microscopy